Merkel cell polyomavirus in non-small cell lung carcinomas from Chile.

Tarik Gheit; Juan Pablo Muñoz; Jorge Levican; Carolina González; Francisco Aguayo; y otros autores.

doi:10.1016/j.yexmp.2012.04.008

Merkel cell polyomavirus in non-small cell lung carcinomas from Chile.

Título traducido de la contribución: Poliomavirus de células de Merkel en carcinomas de pulmón de células no pequeñas de Chile.

Tarik Gheit
, Juan Pablo Muñoz
, Jorge Levican
, Carolina González
, Francisco Aguayo
, y otros autores.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

29 Citas (Scopus)

Resumen

El cáncer de pulmón es una patología importante fuertemente asociada con el hábito de fumar. Sin embargo, se ha sugerido una etiología viral para un subconjunto de pacientes que desarrollan cáncer de pulmón. Los poliomavirus (PyVs) son pequeños virus de ADN bicatenario asociados con el desarrollo de algunas enfermedades humanas. Sin embargo, un papel causal de estos virus en el cáncer humano ha sido difícil de demostrar. En este estudio, ochenta y seis carcinomas de pulmón de células no pequeñas (CPCNP), incluyendo adenocarcinomas (AdCs) y carcinomas de pulmón de células escamosas (SQCs) de Chile fueron analizados para la presencia de PyVs usando reacción en cadena de polimerasa (PCR). Todas las muestras fueron positivas para un fragmento del gen de betaglobina. Encontramos que 4/86 (4.7%) de los carcinomas de pulmón fueron positivos para PyVs. Después de la secuenciación y la alineación BlastN, los cuatro casos fueron identificados como poliomavirus de células de Merkel (MCV) que correspondían a dos AdCs y dos SQCs. Se encontró una asociación estadísticamente no significativa entre la presencia de MCV y las características clinicopatológicas de los pacientes y los tumores. Además, 1/4 (25%) de los carcinomas expresaban activamente transcritos de antígeno T grande (LT), como se demostró mediante PCR con transcriptasa inversa (RT-PCR). Por lo tanto, no se puede descartar un posible papel del MCV en un subgrupo muy pequeño de pacientes con cáncer de pulmón, lo que justifica una mayor investigación.

Título traducido de la contribución	Poliomavirus de células de Merkel en carcinomas de pulmón de células no pequeñas de Chile.
Idioma original	Inglés
Páginas (desde-hasta)	162-166
Publicación	Experimental and Molecular Pathology
Volumen	93
N.º	1
DOI	https://doi.org/10.1016/j.yexmp.2012.04.008
Estado	Publicada - 16 abr. 2012
Publicado de forma externa	Sí

Palabras clave

Cáncer
Pulmón
MCV

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Acceder al documento

10.1016/j.yexmp.2012.04.008

Otros archivos y enlaces

Enlace a la publicación en Scopus

Citar esto

@article{db5a7b00f2184f1e880ee311ab5b29d9,

title = "Merkel cell polyomavirus in non-small cell lung carcinomas from Chile.",

abstract = "Lung cancer is a leading pathology strongly associated with the smoking habit. However, a viral etiology for a subset of patients developing lung cancer has been suggested. Polyomaviruses (PyVs) are small double stranded DNA viruses associated with the development of some human diseases. However, a causal role of these viruses in human cancer has been difficult to demonstrate. In this study, eighty-six non-small cell lung carcinomas (NSCLCs), including adenocarcinomas (AdCs) and squamous cell lung carcinomas (SQCs) from Chile were analyzed for the presence of PyVs using polymerase chain reaction (PCR). All of the specimens were positive for a fragment of the betaglobin gene. We found that 4/86 (4.7\%) of lung carcinomas were positive for PyVs. After sequencing and BlastN alignment, all four cases were identified as Merkel cell polyomaviruses (MCV) that corresponded to two AdCs and two SQCs. A non-significant statistical association was found between the presence of MCV and clinic-pathological features of the patients and tumors. In addition, 1/4 (25\%) of the carcinomas were actively expressing large T antigen (LT) transcripts, as demonstrated by reverse-transcriptase PCR (RT-PCR). Thus a possible role of MCV in a very small subset of patients with lung cancer cannot be ruled out and warrants more investigation. ",

keywords = "C{\'a}ncer, Pulm{\'o}n, MCV, Cancer, Lung, MCV",

author = "Tarik Gheit and Mu{\~n}oz, \{Juan Pablo\} and Jorge Levican and Carolina Gonz{\'a}lez and Francisco Aguayo and \{and other authors.\}",

year = "2012",

month = apr,

day = "16",

doi = "10.1016/j.yexmp.2012.04.008",

language = "English",

volume = "93",

pages = "162--166",

journal = "Experimental and Molecular Pathology",

issn = "0014-4800",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Merkel cell polyomavirus in non-small cell lung carcinomas from Chile.

AU - Gheit, Tarik

AU - Muñoz, Juan Pablo

AU - Levican, Jorge

AU - González, Carolina

AU - Aguayo, Francisco

AU - and other authors.

PY - 2012/4/16

Y1 - 2012/4/16

N2 - Lung cancer is a leading pathology strongly associated with the smoking habit. However, a viral etiology for a subset of patients developing lung cancer has been suggested. Polyomaviruses (PyVs) are small double stranded DNA viruses associated with the development of some human diseases. However, a causal role of these viruses in human cancer has been difficult to demonstrate. In this study, eighty-six non-small cell lung carcinomas (NSCLCs), including adenocarcinomas (AdCs) and squamous cell lung carcinomas (SQCs) from Chile were analyzed for the presence of PyVs using polymerase chain reaction (PCR). All of the specimens were positive for a fragment of the betaglobin gene. We found that 4/86 (4.7%) of lung carcinomas were positive for PyVs. After sequencing and BlastN alignment, all four cases were identified as Merkel cell polyomaviruses (MCV) that corresponded to two AdCs and two SQCs. A non-significant statistical association was found between the presence of MCV and clinic-pathological features of the patients and tumors. In addition, 1/4 (25%) of the carcinomas were actively expressing large T antigen (LT) transcripts, as demonstrated by reverse-transcriptase PCR (RT-PCR). Thus a possible role of MCV in a very small subset of patients with lung cancer cannot be ruled out and warrants more investigation.

AB - Lung cancer is a leading pathology strongly associated with the smoking habit. However, a viral etiology for a subset of patients developing lung cancer has been suggested. Polyomaviruses (PyVs) are small double stranded DNA viruses associated with the development of some human diseases. However, a causal role of these viruses in human cancer has been difficult to demonstrate. In this study, eighty-six non-small cell lung carcinomas (NSCLCs), including adenocarcinomas (AdCs) and squamous cell lung carcinomas (SQCs) from Chile were analyzed for the presence of PyVs using polymerase chain reaction (PCR). All of the specimens were positive for a fragment of the betaglobin gene. We found that 4/86 (4.7%) of lung carcinomas were positive for PyVs. After sequencing and BlastN alignment, all four cases were identified as Merkel cell polyomaviruses (MCV) that corresponded to two AdCs and two SQCs. A non-significant statistical association was found between the presence of MCV and clinic-pathological features of the patients and tumors. In addition, 1/4 (25%) of the carcinomas were actively expressing large T antigen (LT) transcripts, as demonstrated by reverse-transcriptase PCR (RT-PCR). Thus a possible role of MCV in a very small subset of patients with lung cancer cannot be ruled out and warrants more investigation.

KW - Cáncer

KW - Pulmón

KW - MCV

KW - Cancer

KW - Lung

KW - MCV

UR - https://www.scopus.com/pages/publications/84861009870

U2 - 10.1016/j.yexmp.2012.04.008

DO - 10.1016/j.yexmp.2012.04.008

M3 - Article

C2 - 22537543

SN - 0014-4800

VL - 93

SP - 162

EP - 166

JO - Experimental and Molecular Pathology

JF - Experimental and Molecular Pathology

IS - 1

ER -

Merkel cell polyomavirus in non-small cell lung carcinomas from Chile.

Resumen

Palabras clave

ODS de las Naciones Unidas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto