TY - JOUR
T1 - Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species
AU - Muñoz-Rojas, Pablo
AU - Quezada-Romegialli, Claudio
AU - Véliz, David
PY - 2012/6
Y1 - 2012/6
N2 - A total of ten microsatellite loci were isolated and characterized in the endangered freshwater catfish Trichomycterus areolatus from Chile. Samples from three separate watersheds were also analyzed. The mean number of alleles per locus in a sample of 63 individuals was 4 (1-13 alleles per locus) and the observed heterozygosities ranged from 0 to 0.83. Hardy-Weinberg equilibrium was observed in all loci except for a few isolated cases, while none of the loci exhibited significant linkage disequilibrium. Cross-amplification analysis showed a low number of loci and alleles amplified in other seven species of the Family Trichomycteridae.
AB - A total of ten microsatellite loci were isolated and characterized in the endangered freshwater catfish Trichomycterus areolatus from Chile. Samples from three separate watersheds were also analyzed. The mean number of alleles per locus in a sample of 63 individuals was 4 (1-13 alleles per locus) and the observed heterozygosities ranged from 0 to 0.83. Hardy-Weinberg equilibrium was observed in all loci except for a few isolated cases, while none of the loci exhibited significant linkage disequilibrium. Cross-amplification analysis showed a low number of loci and alleles amplified in other seven species of the Family Trichomycteridae.
KW - Cross-amplification
KW - Microsatellite markers
KW - Population genetics
KW - Trichomycterus areolatus
UR - https://www.scopus.com/pages/publications/84892854532
U2 - 10.1007/s12686-011-9569-2
DO - 10.1007/s12686-011-9569-2
M3 - Article
AN - SCOPUS:84892854532
SN - 1877-7252
VL - 4
SP - 443
EP - 445
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -