Interaction between Cigarette Smoke and Human Papillomavirus 16 E6/E7 Oncoproteins to Induce SOD2 Expression and DNA Damage in Head and Neck Cancer.

Diego Carrillo-Beltrán; Julio C. Osorio; Rancés Blanco; Carolina Oliva; Enrique Boccardo; Francisco Aguayo

doi:10.3390/ijms24086907

Interaction between Cigarette Smoke and Human Papillomavirus 16 E6/E7 Oncoproteins to Induce SOD2 Expression and DNA Damage in Head and Neck Cancer.

Título traducido de la contribución: Interacción entre el humo del cigarrillo y las oncoproteínas E6/E7 del virus del papiloma humano 16 para inducir la expresión de SOD2 y daño al ADN en el cáncer de cabeza y cuello.

Diego Carrillo-Beltrán
, Julio C. Osorio
, Rancés Blanco
, Carolina Oliva
, Enrique Boccardo
, Francisco Aguayo

Departamento de Ciencias Biomédicas

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

10 Citas (Scopus)

Resumen

Aunque los estudios epidemiológicos sugieren que el tabaquismo y la infección por el virus del papiloma humano de alto riesgo (VPH-AR) son factores de riesgo mutuamente excluyentes para desarrollar cáncer de cabeza y cuello (CCH), una parte de los sujetos que desarrollan este grupo heterogéneo de cánceres son tanto VPH positivos como fumadores. Ambos factores carcinogénicos están asociados con un aumento del estrés oxidativo (EO) y daño del ADN. Se ha sugerido que la superóxido dismutasa 2 (SOD2) puede ser regulada independientemente por el humo del cigarrillo y el VPH, lo que aumenta la adaptación al EO y la progresión tumoral. En este estudio, analizamos los niveles de SOD2 y el daño del ADN en células orales que expresan ectópicamente las oncoproteínas E6/E7 del VPH16 y expuestas al condensado del humo del cigarrillo (CSC). Además, analizamos las transcripciones de SOD2 en la base de datos de cáncer de cabeza y cuello del Atlas del Genoma del Cáncer (TCGA). Encontramos que las células orales que expresan las oncoproteínas E6/E7 del VPH16 expuestas al CSC aumentaron sinérgicamente los niveles de SOD2 y el daño del ADN. Además, la regulación de la SOD2 por E6 ocurre de forma independiente de Akt1 y ATM. Este estudio sugiere que la interacción entre el VPH y el humo del cigarrillo en el cáncer cervicouterino de cabeza y cuello promueve alteraciones de la SOD2, lo que provoca un mayor daño al ADN y, a su vez, contribuye al desarrollo de una entidad clínica diferente.

Título traducido de la contribución	Interacción entre el humo del cigarrillo y las oncoproteínas E6/E7 del virus del papiloma humano 16 para inducir la expresión de SOD2 y daño al ADN en el cáncer de cabeza y cuello.
Idioma original	Inglés
Número de artículo	6907
Publicación	International Journal of Molecular Sciences
Volumen	24
N.º	8
DOI	https://doi.org/10.3390/ijms24086907
Estado	Publicada - 6 abr. 2023

Palabras clave

Cáncer
Humo de cigarrillo
Daño al ADN
Cabeza y cuello
Virus del papiloma humano
SOD2

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@article{60dfe256e5374a1a851864d55fab6bdd,

title = "Interaction between Cigarette Smoke and Human Papillomavirus 16 E6/E7 Oncoproteins to Induce SOD2 Expression and DNA Damage in Head and Neck Cancer.",

abstract = "Even though epidemiological studies suggest that tobacco smoking and high-risk human papillomavirus (HR-HPV) infection are mutually exclusive risk factors for developing head and neck cancer (HNC), a portion of subjects who develop this heterogeneous group of cancers are both HPV-positive and smokers. Both carcinogenic factors are associated with increased oxidative stress (OS) and DNA damage. It has been suggested that superoxide dismutase 2 (SOD2) can be independently regulated by cigarette smoke and HPV, increasing adaptation to OS and tumor progression. In this study, we analyzed SOD2 levels and DNA damage in oral cells ectopically expressing HPV16 E6/E7 oncoproteins and exposed to cigarette smoke condensate (CSC). Additionally, we analyzed SOD2 transcripts in The Cancer Genome Atlas (TCGA) Head and Neck Cancer Database. We found that oral cells expressing HPV16 E6/E7 oncoproteins exposed to CSC synergistically increased SOD2 levels and DNA damage. Additionally, the SOD2 regulation by E6, occurs in an Akt1 and ATM-independent manner. This study suggests that HPV and cigarette smoke interaction in HNC promotes SOD2 alterations, leading to increased DNA damage and, in turn, contributing to development of a different clinical entity.",

keywords = "C{\'a}ncer, Humo de cigarrillo, Da{\~n}o al ADN, Cabeza y cuello, Virus del papiloma humano, SOD2, Cancer, Cigarette smoke, DNA damage, Head and neck, Human papillomavirus, SOD2",

author = "Diego Carrillo-Beltr{\'a}n and Osorio, \{Julio C.\} and Ranc{\'e}s Blanco and Carolina Oliva and Enrique Boccardo and Francisco Aguayo",

year = "2023",

month = apr,

day = "6",

doi = "10.3390/ijms24086907",

language = "English",

volume = "24",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

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Interaction between Cigarette Smoke and Human Papillomavirus 16 E6/E7 Oncoproteins to Induce SOD2 Expression and DNA Damage in Head and Neck Cancer. / Carrillo-Beltrán, Diego; Osorio, Julio C.; Blanco, Rancés et al.
En: International Journal of Molecular Sciences, Vol. 24, N.º 8, 6907, 06.04.2023.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - Interaction between Cigarette Smoke and Human Papillomavirus 16 E6/E7 Oncoproteins to Induce SOD2 Expression and DNA Damage in Head and Neck Cancer.

AU - Carrillo-Beltrán, Diego

AU - Osorio, Julio C.

AU - Blanco, Rancés

AU - Oliva, Carolina

AU - Boccardo, Enrique

AU - Aguayo, Francisco

PY - 2023/4/6

Y1 - 2023/4/6

N2 - Even though epidemiological studies suggest that tobacco smoking and high-risk human papillomavirus (HR-HPV) infection are mutually exclusive risk factors for developing head and neck cancer (HNC), a portion of subjects who develop this heterogeneous group of cancers are both HPV-positive and smokers. Both carcinogenic factors are associated with increased oxidative stress (OS) and DNA damage. It has been suggested that superoxide dismutase 2 (SOD2) can be independently regulated by cigarette smoke and HPV, increasing adaptation to OS and tumor progression. In this study, we analyzed SOD2 levels and DNA damage in oral cells ectopically expressing HPV16 E6/E7 oncoproteins and exposed to cigarette smoke condensate (CSC). Additionally, we analyzed SOD2 transcripts in The Cancer Genome Atlas (TCGA) Head and Neck Cancer Database. We found that oral cells expressing HPV16 E6/E7 oncoproteins exposed to CSC synergistically increased SOD2 levels and DNA damage. Additionally, the SOD2 regulation by E6, occurs in an Akt1 and ATM-independent manner. This study suggests that HPV and cigarette smoke interaction in HNC promotes SOD2 alterations, leading to increased DNA damage and, in turn, contributing to development of a different clinical entity.

AB - Even though epidemiological studies suggest that tobacco smoking and high-risk human papillomavirus (HR-HPV) infection are mutually exclusive risk factors for developing head and neck cancer (HNC), a portion of subjects who develop this heterogeneous group of cancers are both HPV-positive and smokers. Both carcinogenic factors are associated with increased oxidative stress (OS) and DNA damage. It has been suggested that superoxide dismutase 2 (SOD2) can be independently regulated by cigarette smoke and HPV, increasing adaptation to OS and tumor progression. In this study, we analyzed SOD2 levels and DNA damage in oral cells ectopically expressing HPV16 E6/E7 oncoproteins and exposed to cigarette smoke condensate (CSC). Additionally, we analyzed SOD2 transcripts in The Cancer Genome Atlas (TCGA) Head and Neck Cancer Database. We found that oral cells expressing HPV16 E6/E7 oncoproteins exposed to CSC synergistically increased SOD2 levels and DNA damage. Additionally, the SOD2 regulation by E6, occurs in an Akt1 and ATM-independent manner. This study suggests that HPV and cigarette smoke interaction in HNC promotes SOD2 alterations, leading to increased DNA damage and, in turn, contributing to development of a different clinical entity.

KW - Cáncer

KW - Humo de cigarrillo

KW - Daño al ADN

KW - Cabeza y cuello

KW - Virus del papiloma humano

KW - SOD2

KW - Cancer

KW - Cigarette smoke

KW - DNA damage

KW - Head and neck

KW - Human papillomavirus

KW - SOD2

UR - https://www.scopus.com/pages/publications/85156216373

U2 - 10.3390/ijms24086907

DO - 10.3390/ijms24086907

M3 - Article

C2 - 37108069

SN - 1661-6596

VL - 24

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 8

M1 - 6907

ER -

Interaction between Cigarette Smoke and Human Papillomavirus 16 E6/E7 Oncoproteins to Induce SOD2 Expression and DNA Damage in Head and Neck Cancer.

Resumen

Palabras clave

ODS de las Naciones Unidas

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