Human papillomavirus infection in anal intraepithelial lesions from HIV infected Cuban men.

Celia M. Limia; Yudira Soto; Yanara García; Orestes Blanco; Vivian Kourí; María V. López; María E. Toledo; Lissette Pérez; Yoanna Baños; Yaniris Caturla; Francisco Aguayo

doi:10.1186/s13027-017-0118-9

Human papillomavirus infection in anal intraepithelial lesions from HIV infected Cuban men.

Título traducido de la contribución: Infección por el virus del papiloma humano en lesiones intraepiteliales anales de hombres cubanos infectados con VIH.

Celia M. Limia
, Yudira Soto
, Yanara García
, Orestes Blanco
, Vivian Kourí
, María V. López
, María E. Toledo
, Lissette Pérez
, Yoanna Baños
, Yaniris Caturla
, Francisco Aguayo

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

10 Citas (Scopus)

Resumen

Antecedentes: Se ha establecido una asociación entre la infección por VPH y la progresión a lesiones intraepiteliales escamosas anales (LIEAG), especialmente en poblaciones de alto riesgo como los hombres con VIH. En esta población, el cáncer anal es una de las neoplasias malignas no definitorias de sida más frecuentes. Métodos: Se realizó un estudio transversal para detectar lesiones anales e infección por VPH. Se recolectaron muestras de mucosa anal de 56 hombres con VIH de Cuba. El diagnóstico citológico se realizó según el Sistema Bethesda 2001. La detección del ADN del VPH se determinó mediante qPCR para seis tipos de VPH de alto riesgo y PCR de punto final para los tipos de VPH de bajo riesgo (6 y 11). La PCR de punto final con secuenciación de nucleótidos se empleó para detectar otros genotipos de VPH no incluidos en la qPCR en aquellas muestras negativas para VPH 6 y 11 o negativas para los seis genotipos identificados en la qPCR. Resultados: El diagnóstico citológico identificó citología anal anormal en 53 de 56 hombres (95%). De estos, el 26% (14/53) presentó células escamosas atípicas de significado indeterminado (ASC-US), el 4% (2/53) presentó células escamosas atípicas de significado indeterminado que no permiten descartar lesiones de alto grado (ASC-H), el 64% (34/53) presentó lesiones intraepiteliales escamosas de bajo grado (LIEBG) y el 6% (3/53) presentó lesiones intraepiteliales escamosas de alto grado (LIEAG). Se detectó ADN del VPH en el 89% (50/56) de los hombres y el 79% presentó al menos uno de los tipos de VPH de alto riesgo. El VPH-16 fue el genotipo más frecuente (52%), mientras que el VPH-18 fue el genotipo detectado con mayor frecuencia en hombres con LIEAG. Se encontraron diferencias estadísticamente significativas en las cargas virales del VPH con respecto a los resultados de la citología (p = 0,0006) y se observó que la práctica del sexo anal receptivo era un factor de riesgo para la infección anal por VPH (p = 0,032). Conclusión: Este estudio muestra una alta prevalencia de ASIL e infecciones por VPH de alto riesgo en el grupo estudiado y es el primero en mostrar la distribución de genotipos de VPH en hombres cubanos con VIH y citología anal anormal. Esta información puede ser importante para las autoridades locales a la hora de mejorar las estrategias de prevención, incluyendo la introducción de la vacuna contra el VPH en Cuba.

Título traducido de la contribución	Infección por el virus del papiloma humano en lesiones intraepiteliales anales de hombres cubanos infectados con VIH.
Idioma original	Inglés
Número de artículo	5
Páginas (desde-hasta)	1-8
Número de páginas	8
Publicación	Infectious Agents and Cancer
Volumen	12
N.º	1
DOI	https://doi.org/10.1186/s13027-017-0118-9
Estado	Publicada - 17 ene. 2017
Publicado de forma externa	Sí

Palabras clave

Cáncer anal
Lesiones intraepiteliales escamosas anales
VIH
VPH

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Acceder al documento

10.1186/s13027-017-0118-9Licencia: CC BY

Otros archivos y enlaces

Enlace a la publicación en Scopus

Citar esto

@article{d74f8c9f03004b64857fbf7e8caf6281,

title = "Human papillomavirus infection in anal intraepithelial lesions from HIV infected Cuban men.",

abstract = "Background: An association between HPV infection and progression to anal squamous intraepithelial lesions (ASIL) has been established, specifically in high-risk populations such as HIV-infected men. In this population, anal cancer is one of the most common non-AIDS-defining malignancies. Methods: A cross-sectional study to detect anal lesions and HPV infection was performed. Anal mucosa samples were collected from 56 HIV-infected men from Cuba. The cytological diagnosis was done according to Bethesda 2001 System. HPV DNA detection was determined by qPCR for six high-risk HPV types and end point PCR for low-risk HPV types (6 and 11). The end point PCR with nucleotide sequencing technique was achieved to detect other genotypes of HPV not included in the qPCR in those samples negative for HPV- 6 and 11 or negative for the six genotypes identified in the qPCR. Results: Cytological diagnosis identified 53 of 56 (95\%) men with abnormal anal cytology. Among those, 26\% (14/53) had atypical squamous cells of undetermined significance (ASC-US), 4\% (2/53) had atypical squamous cells of undetermined significance cannot exclude high-grade lesions (ASC-H), 64\% (34/53) had low-grade squamous intraepithelial lesions (LSIL), and 6\% (3/53) had high-grade squamous intraepithelial lesions (HSIL). HPV DNA was detected in 89\% (50/56) of men and 79\% had at least one of the high-risk HPV types. HPV- 16 was the most common genotype (52\%), while HPV-18 was the most frequently detected genotype in men with HSIL. We found statistically significant differences in the HPV viral loads with respect to the cytology results (p = 0.0006) and that the practice of receptive anal sex was a risk factor for anal HPV infection (p = 0.032). Conclusion: This study shows a high prevalence of ASIL and high-risk HPV infections in the study group and is the first study showing the distribution of HPV genotypes in HIV infected Cuban men with abnormal anal cytology. This information may be of importance for local decision makers to improve prevention strategies, including the introduction of HPV vaccine in Cuba.",

keywords = "C{\'a}ncer anal, Lesiones intraepiteliales escamosas anales, VIH, VPH, Anal cancer, Anal squamous intraepithelial lesions, HIV, HPV",

author = "Limia, \{Celia M.\} and Yudira Soto and Yanara Garc{\'i}a and Orestes Blanco and Vivian Kour{\'i} and L{\'o}pez, \{Mar{\'i}a V.\} and Toledo, \{Mar{\'i}a E.\} and Lissette P{\'e}rez and Yoanna Ba{\~n}os and Yaniris Caturla and Francisco Aguayo",

note = "Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = jan,

day = "17",

doi = "10.1186/s13027-017-0118-9",

language = "English",

volume = "12",

pages = "1--8",

journal = "Infectious Agents and Cancer",

issn = "1750-9378",

publisher = "Springer Nature",

number = "1",

}

TY - JOUR

T1 - Human papillomavirus infection in anal intraepithelial lesions from HIV infected Cuban men.

AU - Limia, Celia M.

AU - Soto, Yudira

AU - García, Yanara

AU - Blanco, Orestes

AU - Kourí, Vivian

AU - López, María V.

AU - Toledo, María E.

AU - Pérez, Lissette

AU - Baños, Yoanna

AU - Caturla, Yaniris

AU - Aguayo, Francisco

PY - 2017/1/17

Y1 - 2017/1/17

N2 - Background: An association between HPV infection and progression to anal squamous intraepithelial lesions (ASIL) has been established, specifically in high-risk populations such as HIV-infected men. In this population, anal cancer is one of the most common non-AIDS-defining malignancies. Methods: A cross-sectional study to detect anal lesions and HPV infection was performed. Anal mucosa samples were collected from 56 HIV-infected men from Cuba. The cytological diagnosis was done according to Bethesda 2001 System. HPV DNA detection was determined by qPCR for six high-risk HPV types and end point PCR for low-risk HPV types (6 and 11). The end point PCR with nucleotide sequencing technique was achieved to detect other genotypes of HPV not included in the qPCR in those samples negative for HPV- 6 and 11 or negative for the six genotypes identified in the qPCR. Results: Cytological diagnosis identified 53 of 56 (95%) men with abnormal anal cytology. Among those, 26% (14/53) had atypical squamous cells of undetermined significance (ASC-US), 4% (2/53) had atypical squamous cells of undetermined significance cannot exclude high-grade lesions (ASC-H), 64% (34/53) had low-grade squamous intraepithelial lesions (LSIL), and 6% (3/53) had high-grade squamous intraepithelial lesions (HSIL). HPV DNA was detected in 89% (50/56) of men and 79% had at least one of the high-risk HPV types. HPV- 16 was the most common genotype (52%), while HPV-18 was the most frequently detected genotype in men with HSIL. We found statistically significant differences in the HPV viral loads with respect to the cytology results (p = 0.0006) and that the practice of receptive anal sex was a risk factor for anal HPV infection (p = 0.032). Conclusion: This study shows a high prevalence of ASIL and high-risk HPV infections in the study group and is the first study showing the distribution of HPV genotypes in HIV infected Cuban men with abnormal anal cytology. This information may be of importance for local decision makers to improve prevention strategies, including the introduction of HPV vaccine in Cuba.

AB - Background: An association between HPV infection and progression to anal squamous intraepithelial lesions (ASIL) has been established, specifically in high-risk populations such as HIV-infected men. In this population, anal cancer is one of the most common non-AIDS-defining malignancies. Methods: A cross-sectional study to detect anal lesions and HPV infection was performed. Anal mucosa samples were collected from 56 HIV-infected men from Cuba. The cytological diagnosis was done according to Bethesda 2001 System. HPV DNA detection was determined by qPCR for six high-risk HPV types and end point PCR for low-risk HPV types (6 and 11). The end point PCR with nucleotide sequencing technique was achieved to detect other genotypes of HPV not included in the qPCR in those samples negative for HPV- 6 and 11 or negative for the six genotypes identified in the qPCR. Results: Cytological diagnosis identified 53 of 56 (95%) men with abnormal anal cytology. Among those, 26% (14/53) had atypical squamous cells of undetermined significance (ASC-US), 4% (2/53) had atypical squamous cells of undetermined significance cannot exclude high-grade lesions (ASC-H), 64% (34/53) had low-grade squamous intraepithelial lesions (LSIL), and 6% (3/53) had high-grade squamous intraepithelial lesions (HSIL). HPV DNA was detected in 89% (50/56) of men and 79% had at least one of the high-risk HPV types. HPV- 16 was the most common genotype (52%), while HPV-18 was the most frequently detected genotype in men with HSIL. We found statistically significant differences in the HPV viral loads with respect to the cytology results (p = 0.0006) and that the practice of receptive anal sex was a risk factor for anal HPV infection (p = 0.032). Conclusion: This study shows a high prevalence of ASIL and high-risk HPV infections in the study group and is the first study showing the distribution of HPV genotypes in HIV infected Cuban men with abnormal anal cytology. This information may be of importance for local decision makers to improve prevention strategies, including the introduction of HPV vaccine in Cuba.

KW - Cáncer anal

KW - Lesiones intraepiteliales escamosas anales

KW - VIH

KW - VPH

KW - Anal cancer

KW - Anal squamous intraepithelial lesions

KW - HIV

KW - HPV

UR - https://www.scopus.com/pages/publications/85011256386

U2 - 10.1186/s13027-017-0118-9

DO - 10.1186/s13027-017-0118-9

M3 - Article

C2 - 28115985

SN - 1750-9378

VL - 12

SP - 1

EP - 8

JO - Infectious Agents and Cancer

JF - Infectious Agents and Cancer

IS - 1

M1 - 5

ER -

Human papillomavirus infection in anal intraepithelial lesions from HIV infected Cuban men.

Resumen

Palabras clave

ODS de las Naciones Unidas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto