Human Papillomavirus Detected in Oropharyngeal Cancers from Chilean Subjects

Carolina Oliva; Diego Carrillo-Beltrán; Paul Boettiger; Iván Gallegos; Francisco Aguayo

doi:10.3390/v14061212

Human Papillomavirus Detected in Oropharyngeal Cancers from Chilean Subjects

Título traducido de la contribución: Virus del papiloma humano detectado en cánceres orofaríngeos de pacientes chilenos

Carolina Oliva
, Diego Carrillo-Beltrán
, Paul Boettiger
, Iván Gallegos
, Francisco Aguayo

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

12 Citas (Scopus)

Resumen

Los papilomavirus humanos de alto riesgo (VPH-AR) son los agentes causales de un subgrupo importante de cánceres orofaríngeos cuya incidencia ha aumentado considerablemente en los últimos años. En este estudio, evaluamos la presencia de VPH en 49 casos de cáncer orofaríngeo de pacientes chilenos. La presencia de ADN del VPH se analizó mediante PCR convencional, los genotipos se identificaron mediante secuenciación y la expresión de los transcritos E6/E7 se evaluó mediante reacción en cadena de la polimerasa con transcriptasa inversa (RT-PCR). Además, para determinar la expresión de p16 —un marcador indirecto de la infección por VPH oncogénico— se construyó una matriz tisular para inmunohistoquímica (IHQ). Se detectó VPH en el 61,2 % de los carcinomas orofaríngeos, siendo el genotipo más prevalente el VPH16 (80 %). Los transcritos E6 y E7 se detectaron en el 91,6 % y el 79,1 % de las muestras positivas para VPH16, respectivamente, lo que demuestra infecciones funcionales por VPH. Además, la expresión de p16 fue positiva en el 58,3 % de los casos. Estos hallazgos demuestran una alta prevalencia de VPH de alto riesgo en tumores orofaríngeos de Chile, lo que sugiere la necesidad de realizar estudios adicionales para abordar este creciente problema de salud pública.

Título traducido de la contribución	Virus del papiloma humano detectado en cánceres orofaríngeos de pacientes chilenos
Idioma original	Inglés
Número de artículo	1212
Publicación	Viruses
Volumen	14
N.º	6
DOI	https://doi.org/10.3390/v14061212
Estado	Publicada - 1 jun. 2022
Publicado de forma externa	Sí

Palabras clave

Cabeza y cuello
Virus del papiloma humano
Cáncer orofaríngeo
Carcinomas de células escamosas (OPSCC)

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@article{c679b438156e4ac29f5ac81448b3d30c,

title = "Human Papillomavirus Detected in Oropharyngeal Cancers from Chilean Subjects",

abstract = "High-risk human papillomaviruses (HR-HPV) are the causal agents of an important subset of oropharyngeal cancers that has increased considerably in incidence in recent years. In this study, we evaluated the presence of HPV in 49 oropharyngeal cancers from Chilean subjects. The presence of HPV DNA was analyzed by conventional PCR, the genotypes were identified through sequencing, and the expression of E6/E7 transcripts was evaluated by a reverse transcriptase polymerase chain reaction (RT-PCR). Additionally, to determine p16 expression—a surrogate marker for oncogenic HPV infection—a tissue array was constructed for immunohistochemistry (IHC). HPV was detected in 61.2\% of oropharyngeal carcinomas, the most prevalent genotype being HPV16 (80\%). E6 and E7 transcripts were detected in 91.6\% and 79.1\% of the HPV16-positive specimens, respectively, demonstrating functional HPV infections. Furthermore, p16 expression was positive in 58.3\% of cases. These findings show a high prevalence of HR-HPV in oropharyngeal tumors from Chile, suggesting the necessity of additional studies to address this growing public health concern.",

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author = "Carolina Oliva and Diego Carrillo-Beltr{\'a}n and Paul Boettiger and Iv{\'a}n Gallegos and Francisco Aguayo",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = jun,

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doi = "10.3390/v14061212",

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AU - Oliva, Carolina

AU - Carrillo-Beltrán, Diego

AU - Boettiger, Paul

AU - Gallegos, Iván

AU - Aguayo, Francisco

PY - 2022/6/1

Y1 - 2022/6/1

N2 - High-risk human papillomaviruses (HR-HPV) are the causal agents of an important subset of oropharyngeal cancers that has increased considerably in incidence in recent years. In this study, we evaluated the presence of HPV in 49 oropharyngeal cancers from Chilean subjects. The presence of HPV DNA was analyzed by conventional PCR, the genotypes were identified through sequencing, and the expression of E6/E7 transcripts was evaluated by a reverse transcriptase polymerase chain reaction (RT-PCR). Additionally, to determine p16 expression—a surrogate marker for oncogenic HPV infection—a tissue array was constructed for immunohistochemistry (IHC). HPV was detected in 61.2% of oropharyngeal carcinomas, the most prevalent genotype being HPV16 (80%). E6 and E7 transcripts were detected in 91.6% and 79.1% of the HPV16-positive specimens, respectively, demonstrating functional HPV infections. Furthermore, p16 expression was positive in 58.3% of cases. These findings show a high prevalence of HR-HPV in oropharyngeal tumors from Chile, suggesting the necessity of additional studies to address this growing public health concern.

AB - High-risk human papillomaviruses (HR-HPV) are the causal agents of an important subset of oropharyngeal cancers that has increased considerably in incidence in recent years. In this study, we evaluated the presence of HPV in 49 oropharyngeal cancers from Chilean subjects. The presence of HPV DNA was analyzed by conventional PCR, the genotypes were identified through sequencing, and the expression of E6/E7 transcripts was evaluated by a reverse transcriptase polymerase chain reaction (RT-PCR). Additionally, to determine p16 expression—a surrogate marker for oncogenic HPV infection—a tissue array was constructed for immunohistochemistry (IHC). HPV was detected in 61.2% of oropharyngeal carcinomas, the most prevalent genotype being HPV16 (80%). E6 and E7 transcripts were detected in 91.6% and 79.1% of the HPV16-positive specimens, respectively, demonstrating functional HPV infections. Furthermore, p16 expression was positive in 58.3% of cases. These findings show a high prevalence of HR-HPV in oropharyngeal tumors from Chile, suggesting the necessity of additional studies to address this growing public health concern.

KW - Cabeza y cuello

KW - Virus del papiloma humano

KW - Cáncer orofaríngeo

KW - Carcinomas de células escamosas (OPSCC)

KW - Head and neck

KW - Human papillomavirus

KW - Oropharyngeal cancer

KW - Squamous cell carcinomas (OPSCC)

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U2 - 10.3390/v14061212

DO - 10.3390/v14061212

M3 - Article

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SN - 1999-4915

VL - 14

JO - Viruses

JF - Viruses

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ER -

Human Papillomavirus Detected in Oropharyngeal Cancers from Chilean Subjects

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ODS de las Naciones Unidas

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