Human Papillomavirus, Cytomegalovirus Infection and P16 Staining in Breast Tumors from Peruvian Women.

Gabriela Calderon; Carlos A Castaneda; Miluska Castillo; Joselyn Sanchez; Luis Bernabe; Nancy Suarez; Katherine Tello; Ebert Torres; Jose M Cotrina; Jorge Dunstan; Miguel De-La-Cruz; Julio Abugattas; Henry Guerra; Javier E Manrique; Francisco Renán Aguayo-González; Henry L Gomez

doi:10.31557/apjcp.2022.23.5.1571

Human Papillomavirus, Cytomegalovirus Infection and P16 Staining in Breast Tumors from Peruvian Women.

Título traducido de la contribución: Infección por Virus del Papiloma Humano, Citomegalovirus y Tinción P16 en Tumores de Mama de Mujeres Peruanas.

Gabriela Calderon
, Carlos A Castaneda
, Miluska Castillo
, Joselyn Sanchez
, Luis Bernabe
, Nancy Suarez
, Katherine Tello
, Ebert Torres
, Jose M Cotrina
, Jorge Dunstan
, Miguel De-La-Cruz
, Julio Abugattas
, Henry Guerra
, Javier E Manrique
, Francisco Renán Aguayo-González
, Henry L Gomez

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

8 Citas (Scopus)

Resumen

Objetivo: Evaluar la distribución de frecuencias de infecciones virales en lesiones de cáncer de mama (CM) peruano y su asociación con características clinicopatológicas. Adicionalmente, se realizó una evaluación prospectiva de los niveles de p16 y linfocitos infiltrantes de tumor (TIL) para desarrollar un análisis integral. Métodos: Se realizó detección de virus de papiloma humano de alto riesgo (VPH-AR) mediante qPCR en 447 CM y 79 muestras congeladas no cancerosas. Muestras pareadas de parafina de 238 CM se tiñeron con citomegalovirus humano (CMVH) e inmunohistoquímica de p16. Se calculó TIL en 397 casos de CM. Resultados: El CMVH fue positivo en el 72,5%. Se detectó VPH-AR en el 2,9% de los CM y en el 1,3% de las muestras no malignas. Se encontró P16+ en el 28,15% y la mediana del porcentaje de TIL fue de 30. La infección por VPH de alto riesgo se asoció con histología no ductal (p = 0,003) y p16+ (p = 0,017). Un resultado positivo de P16+ se asoció con un estadio T más alto (p = 0,022), grado (p = 0,009), nivel de TIL (p = 0,002) y fenotipo triple negativo (p = 0,021). Conclusión: El HCMV es frecuente, pero la infección por VPH de alto riesgo es inusual en el BC peruano. P16+ se asocia con infección por PVH de alto riesgo, TIL elevado y características agresivas.

Título traducido de la contribución	Infección por Virus del Papiloma Humano, Citomegalovirus y Tinción P16 en Tumores de Mama de Mujeres Peruanas.
Idioma original	Inglés
Publicación	Asian Pacific journal of cancer prevention : APJCP
Volumen	23
N.º	5
DOI	https://doi.org/10.31557/apjcp.2022.23.5.1571
Estado	Publicada - may. 2022
Publicado de forma externa	Sí

Palabras clave

Virus del papiloma humano
Citomegalovirus
Proteína p16
Reacción en cadena de la polimerasa
Inmunohistoquímica

ODS de las Naciones Unidas

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Calderon, G., Castaneda, C. A., Castillo, M., Sanchez, J., Bernabe, L., Suarez, N., Tello, K., Torres, E., Cotrina, J. M., Dunstan, J., De-La-Cruz, M., Abugattas, J., Guerra, H., Manrique, J. E., Aguayo-González, F. R., & Gomez, H. L. (2022). Human Papillomavirus, Cytomegalovirus Infection and P16 Staining in Breast Tumors from Peruvian Women. Asian Pacific journal of cancer prevention : APJCP, 23(5). https://doi.org/10.31557/apjcp.2022.23.5.1571

@article{fa1173067c6941cf9bc212b135186d57,

title = "Human Papillomavirus, Cytomegalovirus Infection and P16 Staining in Breast Tumors from Peruvian Women.",

abstract = "Objective: To evaluate the frequency distribution of viral infections in Peruvian Breast Cancer (BC) lesions and its association with clinicopathological features. Additionally, a prospective evaluation of p16 and Tumor-infiltrating lymphocytes (TIL) levels were performed for developing a comprehensive analysis. Methods: Detection of high risk- human papillomavirus (HR- HPV) through qPCR was performed in 447 BC and 79 non-cancer frozen samples. Paired paraffin samples from 238 BC were stained with Human cytomegalovirus (HCMV) and p16 immunohistochemistry. TIL was calculated in 397 BC cases. Results: HCMV was positive in 72.5\%. HR- HPV was detected in 2.9\% of BC and 1.3\% of non-malignant samples. P16+ was found in 28.15\% and median TIL percentage was 30. HR- HPV infection was associated with non-ductal histology (p=0.003) and p16+ (p=0.017). Positive P16+ was associated with higher T stage (p=0.022), grade (p=0.009), TIL level (p=0.002), and triple-negative phenotype (p=0.021). Conclusion: HCMV is frequent, but HR- HPV infection is unusual in Peruvian BC. P16+ is associated with HR- PVH infection, high TIL and aggressive features. ",

keywords = "Virus del papiloma humano, Citomegalovirus, Prote{\'i}na p16, Reacci{\'o}n en cadena de la polimerasa, Inmunohistoqu{\'i}mica, Human papillomavirus, Cytomegalovirus, P16 protein, Polymerase chain reaction, Immunohistochemistry",

author = "Gabriela Calderon and Castaneda, \{Carlos A\} and Miluska Castillo and Joselyn Sanchez and Luis Bernabe and Nancy Suarez and Katherine Tello and Ebert Torres and Cotrina, \{Jose M\} and Jorge Dunstan and Miguel De-La-Cruz and Julio Abugattas and Henry Guerra and Manrique, \{Javier E\} and Aguayo-Gonz{\'a}lez, \{Francisco Ren{\'a}n\} and Gomez, \{Henry L\}",

year = "2022",

month = may,

doi = "10.31557/apjcp.2022.23.5.1571",

language = "English",

volume = "23",

journal = "Asian Pacific journal of cancer prevention : APJCP",

number = "5",

}

Calderon, G, Castaneda, CA, Castillo, M, Sanchez, J, Bernabe, L, Suarez, N, Tello, K, Torres, E, Cotrina, JM, Dunstan, J, De-La-Cruz, M, Abugattas, J, Guerra, H, Manrique, JE, Aguayo-González, FR & Gomez, HL 2022, 'Human Papillomavirus, Cytomegalovirus Infection and P16 Staining in Breast Tumors from Peruvian Women.', Asian Pacific journal of cancer prevention : APJCP, vol. 23, n.º 5. https://doi.org/10.31557/apjcp.2022.23.5.1571

TY - JOUR

T1 - Human Papillomavirus, Cytomegalovirus Infection and P16 Staining in Breast Tumors from Peruvian Women.

AU - Calderon, Gabriela

AU - Castaneda, Carlos A

AU - Castillo, Miluska

AU - Sanchez, Joselyn

AU - Bernabe, Luis

AU - Suarez, Nancy

AU - Tello, Katherine

AU - Torres, Ebert

AU - Cotrina, Jose M

AU - Dunstan, Jorge

AU - De-La-Cruz, Miguel

AU - Abugattas, Julio

AU - Guerra, Henry

AU - Manrique, Javier E

AU - Aguayo-González, Francisco Renán

AU - Gomez, Henry L

PY - 2022/5

Y1 - 2022/5

N2 - Objective: To evaluate the frequency distribution of viral infections in Peruvian Breast Cancer (BC) lesions and its association with clinicopathological features. Additionally, a prospective evaluation of p16 and Tumor-infiltrating lymphocytes (TIL) levels were performed for developing a comprehensive analysis. Methods: Detection of high risk- human papillomavirus (HR- HPV) through qPCR was performed in 447 BC and 79 non-cancer frozen samples. Paired paraffin samples from 238 BC were stained with Human cytomegalovirus (HCMV) and p16 immunohistochemistry. TIL was calculated in 397 BC cases. Results: HCMV was positive in 72.5%. HR- HPV was detected in 2.9% of BC and 1.3% of non-malignant samples. P16+ was found in 28.15% and median TIL percentage was 30. HR- HPV infection was associated with non-ductal histology (p=0.003) and p16+ (p=0.017). Positive P16+ was associated with higher T stage (p=0.022), grade (p=0.009), TIL level (p=0.002), and triple-negative phenotype (p=0.021). Conclusion: HCMV is frequent, but HR- HPV infection is unusual in Peruvian BC. P16+ is associated with HR- PVH infection, high TIL and aggressive features.

AB - Objective: To evaluate the frequency distribution of viral infections in Peruvian Breast Cancer (BC) lesions and its association with clinicopathological features. Additionally, a prospective evaluation of p16 and Tumor-infiltrating lymphocytes (TIL) levels were performed for developing a comprehensive analysis. Methods: Detection of high risk- human papillomavirus (HR- HPV) through qPCR was performed in 447 BC and 79 non-cancer frozen samples. Paired paraffin samples from 238 BC were stained with Human cytomegalovirus (HCMV) and p16 immunohistochemistry. TIL was calculated in 397 BC cases. Results: HCMV was positive in 72.5%. HR- HPV was detected in 2.9% of BC and 1.3% of non-malignant samples. P16+ was found in 28.15% and median TIL percentage was 30. HR- HPV infection was associated with non-ductal histology (p=0.003) and p16+ (p=0.017). Positive P16+ was associated with higher T stage (p=0.022), grade (p=0.009), TIL level (p=0.002), and triple-negative phenotype (p=0.021). Conclusion: HCMV is frequent, but HR- HPV infection is unusual in Peruvian BC. P16+ is associated with HR- PVH infection, high TIL and aggressive features.

KW - Virus del papiloma humano

KW - Citomegalovirus

KW - Proteína p16

KW - Reacción en cadena de la polimerasa

KW - Inmunohistoquímica

KW - Human papillomavirus

KW - Cytomegalovirus

KW - P16 protein

KW - Polymerase chain reaction

KW - Immunohistochemistry

UR - https://www.scopus.com/pages/publications/85131108538

U2 - 10.31557/apjcp.2022.23.5.1571

DO - 10.31557/apjcp.2022.23.5.1571

M3 - Article

C2 - 35633540

VL - 23

JO - Asian Pacific journal of cancer prevention : APJCP

JF - Asian Pacific journal of cancer prevention : APJCP

IS - 5

ER -

Human Papillomavirus, Cytomegalovirus Infection and P16 Staining in Breast Tumors from Peruvian Women.

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