Effect of biopesticides and Manure on Tylenchulus semipenetrans cobb in a conventional olive orchard (Olea europaea L.) in the Azapa valley, Chile

One of the most important pests which affects the olive orchards in the Azapa valley is the citrus nematode Tylenchulus semipenetrans Cobb. The main objective of this study was to evaluate the effect to compounds of biologic origin on the populations of T. semipenetrans Cobb in an olive orchard (Olea europaea). The experiment was carried out in an olive orchard in the Azapa valley, Arica, Parinacota region, Chile. For the experimental design four complete randomized blocks were used. There were five treatments and one untreated control, with four replications: Manure (T1), Micosplag® WP (T2), QL Agri® 35 (T3), DiTera® WG (T4), Nemacur® 240 CS (T5) and untreated control (T0). We evaluated the populations of the mobile second stage (J2) and adult females of T. semipenetrans at 120 days after application of treatments. The effectiveness of treatments was determined using the Abbott formula ((living individuals in the control - living individuals in the treatment)/ living individuals in the control) × 100) and the Oostenbrink reproduction factor (R) which uses initial (Pi) and final (Pf) populations (R = Pf/Pi) to estimate reproductive capabilities of nematodes. Data were analyzed using ANOVA and the Duncan test (p≤0.01) was used for significant differences between treatments. The evaluations demonstrated that applications of biological products and manure can control the citrus nematode, and DiTera WG was as effective (p≤0.01) as a chemical product Nemacur 240.