A fast and efficient method for total DNA extraction from soil filamentous fungi

Wilson Huanca-Mamani; Ricardo Salvatierra Martínez; Germán Sepúlveda-Chavera

doi:10.4067/S0718-34292014000200010

A fast and efficient method for total DNA extraction from soil filamentous fungi

Título traducido de la contribución: Un método rápido y eficiente para la extracción de ADN total de hongos filamentosos del suelo

Wilson Huanca-Mamani
, Ricardo Salvatierra Martínez
, Germán Sepúlveda-Chavera

Departamento de Recursos Ambientales

Universidad de Tarapacá

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

7 Citas (Scopus)

Resumen

Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.

Título traducido de la contribución	Un método rápido y eficiente para la extracción de ADN total de hongos filamentosos del suelo
Idioma original	Inglés
Páginas (desde-hasta)	75-78
Número de páginas	4
Publicación	Idesia
Volumen	32
N.º	2
DOI	https://doi.org/10.4067/S0718-34292014000200010
Estado	Publicada - may. 2014

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title = "A fast and efficient method for total DNA extraction from soil filamentous fungi",

abstract = "Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.",

keywords = "DNA extraction, Fungi, PCR, Soil",

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AU - Huanca-Mamani, Wilson

AU - Salvatierra Martínez, Ricardo

AU - Sepúlveda-Chavera, Germán

PY - 2014/5

Y1 - 2014/5

N2 - Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.

AB - Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.

KW - DNA extraction

KW - Fungi

KW - PCR

KW - Soil

UR - https://www.scopus.com/pages/publications/84903436961

U2 - 10.4067/S0718-34292014000200010

DO - 10.4067/S0718-34292014000200010

M3 - Article

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VL - 32

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A fast and efficient method for total DNA extraction from soil filamentous fungi

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