Tropomyosin allergen from freshwater and marine water crabs: Identification, structural-physicochemical, rheological and allergenic characterization

Omgy Neha; Anandan Rangasamy; Sankar T․V․; Vijay Kumar Reddy Surasani; Lalitha Gnanasekaran; Saranya Vinayagam; Thanigaivel Sundaram; Ranjana Devi Mutum; Turki Kh Faraj; Abdulwahed Fahad Alrefaei; Pavan Kumar Dara; Maximilian Lackner

doi:10.1016/j.afres.2025.100894

Tropomyosin allergen from freshwater and marine water crabs: Identification, structural-physicochemical, rheological and allergenic characterization

Omgy Neha
, Anandan Rangasamy
, Sankar T․V․
, Vijay Kumar Reddy Surasani
, Lalitha Gnanasekaran
, Saranya Vinayagam
, Thanigaivel Sundaram
, Ranjana Devi Mutum
, Turki Kh Faraj
, Abdulwahed Fahad Alrefaei
, Pavan Kumar Dara
, Maximilian Lackner

Department of Mechanical Engineering

SCMS-School of Technology and Management
ICAR - Central Institute of Fisheries Technology, Cochin
Guru Angad Dev Veterinary and Animal Sciences University
Saveetha Institute of Medical and Technical Sciences (Deemed to be University)
SRM Institute of Science and Technology
Institute of Bioresources and Sustainable Development
King Saud University
University of Applied Sciences Technikum Wien

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The aim of the present investigation was to purify and characterize highly allergenic tropomyosin from economically important crab species of freshwater and marine sources. Among the six crab species, the highest level of tropomyosin was detected in the raw samples of Scylla olivacea (5.68 ± 0.2 mg/g), whereas the lowest level was detected in the raw samples of Charybdis ferriatus (3.13 ± 0.2 mg/g). The electrophoretic analysis of purified tropomyosin showed the presence of bands within the range of 32–38 kDa. The purified tropomyosin obtained from S. olivacea (freshwater) and C. ferriatus (marine provenience) was found to have lower allergenicity threshold levels of 238.93 ppb and 225.58 ppb as revealed by enzyme-linked immunosorbent assays (ELISA). The tropomyosin with more allergenicity was further characterized for its structural, physico-biochemical, and thermal properties using Fourier transform infrared (FT-IR) spectroscopy, high-performance liquid chromatography (HPLC), and thermogravimetric analysis (TGA). FTIR revealed the presence of arginine in the allergen tropomyosin which plays an important role in eliciting allergenicity. TGA confirmed better stability in the crab allergen tropomyosin compared to that of standard tropomyosin. The flow behavior studies of allergenic tropomyosin revealed less viscous and shear-thickening behavior.

Original language	English
Article number	100894
Journal	Applied Food Research
Volume	5
Issue number	1
DOIs	https://doi.org/10.1016/j.afres.2025.100894
State	Published - Jun 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Allergen
Crab
FTIR
TGA
Tropomyosin

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10.1016/j.afres.2025.100894

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Neha, O., Rangasamy, A., T․V․, S., Surasani, V. K. R., Gnanasekaran, L., Vinayagam, S., Sundaram, T., Mutum, R. D., Faraj, T. K., Alrefaei, A. F., Dara, P. K., & Lackner, M. (2025). Tropomyosin allergen from freshwater and marine water crabs: Identification, structural-physicochemical, rheological and allergenic characterization. Applied Food Research, 5(1), Article 100894. https://doi.org/10.1016/j.afres.2025.100894

@article{dd021e25221b4b0cb1123aad56210325,

title = "Tropomyosin allergen from freshwater and marine water crabs: Identification, structural-physicochemical, rheological and allergenic characterization",

abstract = "The aim of the present investigation was to purify and characterize highly allergenic tropomyosin from economically important crab species of freshwater and marine sources. Among the six crab species, the highest level of tropomyosin was detected in the raw samples of Scylla olivacea (5.68 ± 0.2 mg/g), whereas the lowest level was detected in the raw samples of Charybdis ferriatus (3.13 ± 0.2 mg/g). The electrophoretic analysis of purified tropomyosin showed the presence of bands within the range of 32–38 kDa. The purified tropomyosin obtained from S. olivacea (freshwater) and C. ferriatus (marine provenience) was found to have lower allergenicity threshold levels of 238.93 ppb and 225.58 ppb as revealed by enzyme-linked immunosorbent assays (ELISA). The tropomyosin with more allergenicity was further characterized for its structural, physico-biochemical, and thermal properties using Fourier transform infrared (FT-IR) spectroscopy, high-performance liquid chromatography (HPLC), and thermogravimetric analysis (TGA). FTIR revealed the presence of arginine in the allergen tropomyosin which plays an important role in eliciting allergenicity. TGA confirmed better stability in the crab allergen tropomyosin compared to that of standard tropomyosin. The flow behavior studies of allergenic tropomyosin revealed less viscous and shear-thickening behavior.",

keywords = "Allergen, Crab, FTIR, TGA, Tropomyosin",

author = "Omgy Neha and Anandan Rangasamy and Sankar T․V․ and Surasani, \{Vijay Kumar Reddy\} and Lalitha Gnanasekaran and Saranya Vinayagam and Thanigaivel Sundaram and Mutum, \{Ranjana Devi\} and Faraj, \{Turki Kh\} and Alrefaei, \{Abdulwahed Fahad\} and Dara, \{Pavan Kumar\} and Maximilian Lackner",

note = "Publisher Copyright: {\textcopyright} 2025 The Authors",

year = "2025",

month = jun,

doi = "10.1016/j.afres.2025.100894",

language = "English",

volume = "5",

journal = "Applied Food Research",

issn = "2772-5022",

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Neha, O, Rangasamy, A, T․V․, S, Surasani, VKR, Gnanasekaran, L, Vinayagam, S, Sundaram, T, Mutum, RD, Faraj, TK, Alrefaei, AF, Dara, PK & Lackner, M 2025, 'Tropomyosin allergen from freshwater and marine water crabs: Identification, structural-physicochemical, rheological and allergenic characterization', Applied Food Research, vol. 5, no. 1, 100894. https://doi.org/10.1016/j.afres.2025.100894

TY - JOUR

T1 - Tropomyosin allergen from freshwater and marine water crabs

T2 - Identification, structural-physicochemical, rheological and allergenic characterization

AU - Neha, Omgy

AU - Rangasamy, Anandan

AU - T․V․, Sankar

AU - Surasani, Vijay Kumar Reddy

AU - Gnanasekaran, Lalitha

AU - Vinayagam, Saranya

AU - Sundaram, Thanigaivel

AU - Mutum, Ranjana Devi

AU - Faraj, Turki Kh

AU - Alrefaei, Abdulwahed Fahad

AU - Dara, Pavan Kumar

AU - Lackner, Maximilian

PY - 2025/6

Y1 - 2025/6

N2 - The aim of the present investigation was to purify and characterize highly allergenic tropomyosin from economically important crab species of freshwater and marine sources. Among the six crab species, the highest level of tropomyosin was detected in the raw samples of Scylla olivacea (5.68 ± 0.2 mg/g), whereas the lowest level was detected in the raw samples of Charybdis ferriatus (3.13 ± 0.2 mg/g). The electrophoretic analysis of purified tropomyosin showed the presence of bands within the range of 32–38 kDa. The purified tropomyosin obtained from S. olivacea (freshwater) and C. ferriatus (marine provenience) was found to have lower allergenicity threshold levels of 238.93 ppb and 225.58 ppb as revealed by enzyme-linked immunosorbent assays (ELISA). The tropomyosin with more allergenicity was further characterized for its structural, physico-biochemical, and thermal properties using Fourier transform infrared (FT-IR) spectroscopy, high-performance liquid chromatography (HPLC), and thermogravimetric analysis (TGA). FTIR revealed the presence of arginine in the allergen tropomyosin which plays an important role in eliciting allergenicity. TGA confirmed better stability in the crab allergen tropomyosin compared to that of standard tropomyosin. The flow behavior studies of allergenic tropomyosin revealed less viscous and shear-thickening behavior.

AB - The aim of the present investigation was to purify and characterize highly allergenic tropomyosin from economically important crab species of freshwater and marine sources. Among the six crab species, the highest level of tropomyosin was detected in the raw samples of Scylla olivacea (5.68 ± 0.2 mg/g), whereas the lowest level was detected in the raw samples of Charybdis ferriatus (3.13 ± 0.2 mg/g). The electrophoretic analysis of purified tropomyosin showed the presence of bands within the range of 32–38 kDa. The purified tropomyosin obtained from S. olivacea (freshwater) and C. ferriatus (marine provenience) was found to have lower allergenicity threshold levels of 238.93 ppb and 225.58 ppb as revealed by enzyme-linked immunosorbent assays (ELISA). The tropomyosin with more allergenicity was further characterized for its structural, physico-biochemical, and thermal properties using Fourier transform infrared (FT-IR) spectroscopy, high-performance liquid chromatography (HPLC), and thermogravimetric analysis (TGA). FTIR revealed the presence of arginine in the allergen tropomyosin which plays an important role in eliciting allergenicity. TGA confirmed better stability in the crab allergen tropomyosin compared to that of standard tropomyosin. The flow behavior studies of allergenic tropomyosin revealed less viscous and shear-thickening behavior.

KW - Allergen

KW - Crab

KW - FTIR

KW - TGA

KW - Tropomyosin

UR - https://www.scopus.com/pages/publications/105002674655

U2 - 10.1016/j.afres.2025.100894

DO - 10.1016/j.afres.2025.100894

M3 - Article

AN - SCOPUS:105002674655

SN - 2772-5022

VL - 5

JO - Applied Food Research

JF - Applied Food Research

IS - 1

M1 - 100894

ER -

Tropomyosin allergen from freshwater and marine water crabs: Identification, structural-physicochemical, rheological and allergenic characterization

Abstract

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Keywords

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