Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species

Pablo Muñoz-Rojas; Claudio Quezada-Romegialli; David Véliz

doi:10.1007/s12686-011-9569-2

Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species

Pablo Muñoz-Rojas
, Claudio Quezada-Romegialli
, David Véliz

Universidad de Chile

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A total of ten microsatellite loci were isolated and characterized in the endangered freshwater catfish Trichomycterus areolatus from Chile. Samples from three separate watersheds were also analyzed. The mean number of alleles per locus in a sample of 63 individuals was 4 (1-13 alleles per locus) and the observed heterozygosities ranged from 0 to 0.83. Hardy-Weinberg equilibrium was observed in all loci except for a few isolated cases, while none of the loci exhibited significant linkage disequilibrium. Cross-amplification analysis showed a low number of loci and alleles amplified in other seven species of the Family Trichomycteridae.

Original language	English
Pages (from-to)	443-445
Number of pages	3
Journal	Conservation Genetics Resources
Volume	4
Issue number	2
DOIs	https://doi.org/10.1007/s12686-011-9569-2
State	Published - Jun 2012
Externally published	Yes

Keywords

Cross-amplification
Microsatellite markers
Population genetics
Trichomycterus areolatus

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10.1007/s12686-011-9569-2

Cite this

@article{d961124e7cf740dc84fd87a266fa665f,

title = "Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species",

abstract = "A total of ten microsatellite loci were isolated and characterized in the endangered freshwater catfish Trichomycterus areolatus from Chile. Samples from three separate watersheds were also analyzed. The mean number of alleles per locus in a sample of 63 individuals was 4 (1-13 alleles per locus) and the observed heterozygosities ranged from 0 to 0.83. Hardy-Weinberg equilibrium was observed in all loci except for a few isolated cases, while none of the loci exhibited significant linkage disequilibrium. Cross-amplification analysis showed a low number of loci and alleles amplified in other seven species of the Family Trichomycteridae.",

keywords = "Cross-amplification, Microsatellite markers, Population genetics, Trichomycterus areolatus",

author = "Pablo Mu{\~n}oz-Rojas and Claudio Quezada-Romegialli and David V{\'e}liz",

year = "2012",

month = jun,

doi = "10.1007/s12686-011-9569-2",

language = "English",

volume = "4",

pages = "443--445",

journal = "Conservation Genetics Resources",

issn = "1877-7252",

number = "2",

}

Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species. / Muñoz-Rojas, Pablo; Quezada-Romegialli, Claudio; Véliz, David.
In: Conservation Genetics Resources, Vol. 4, No. 2, 06.2012, p. 443-445.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species

AU - Muñoz-Rojas, Pablo

AU - Quezada-Romegialli, Claudio

AU - Véliz, David

PY - 2012/6

Y1 - 2012/6

N2 - A total of ten microsatellite loci were isolated and characterized in the endangered freshwater catfish Trichomycterus areolatus from Chile. Samples from three separate watersheds were also analyzed. The mean number of alleles per locus in a sample of 63 individuals was 4 (1-13 alleles per locus) and the observed heterozygosities ranged from 0 to 0.83. Hardy-Weinberg equilibrium was observed in all loci except for a few isolated cases, while none of the loci exhibited significant linkage disequilibrium. Cross-amplification analysis showed a low number of loci and alleles amplified in other seven species of the Family Trichomycteridae.

AB - A total of ten microsatellite loci were isolated and characterized in the endangered freshwater catfish Trichomycterus areolatus from Chile. Samples from three separate watersheds were also analyzed. The mean number of alleles per locus in a sample of 63 individuals was 4 (1-13 alleles per locus) and the observed heterozygosities ranged from 0 to 0.83. Hardy-Weinberg equilibrium was observed in all loci except for a few isolated cases, while none of the loci exhibited significant linkage disequilibrium. Cross-amplification analysis showed a low number of loci and alleles amplified in other seven species of the Family Trichomycteridae.

KW - Cross-amplification

KW - Microsatellite markers

KW - Population genetics

KW - Trichomycterus areolatus

UR - https://www.scopus.com/pages/publications/84892854532

U2 - 10.1007/s12686-011-9569-2

DO - 10.1007/s12686-011-9569-2

M3 - Article

AN - SCOPUS:84892854532

SN - 1877-7252

VL - 4

SP - 443

EP - 445

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 2

ER -

Isolation and characterization of ten microsatellite loci in the catfish Trichomycterus areolatus (Siluriformes: Trichomycteridae), with cross-amplification in seven Trichomycterinae species

Abstract

Keywords

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