Immobilization of enzymes for bioremediation: A future remedial and mitigating strategy

Over the years, extensive urbanization and industrialization have led to xenobiotics contamination of the environment and also posed a severe threat to human health. Although there are multiple physical and chemical techniques for xenobiotic pollutants management, bioremediation seems to be a promising technology from the environmental perspective. It is an eco-friendly and low-cost method involving the application of microbes, plants, or their enzymes to degrade xenobiotics into less toxic or non-toxic forms. Moreover, bioremediation involving enzymes has gained an advantage over microorganisms or phytoremediation due to better activity for pollutant degradation with less waste generation. However, the significant disadvantages associated with the application of enzymes are low stability (storage, pH, and temperature) as well as the low possibility of reuse as it is hard to separate from reaction media. The immobilization of enzymes without affecting their activity provides a possible solution to the problems and allows reusability by easing the process of separation with improved stability to various environmental factors. The present communication provides an overview of the importance of enzyme immobilization in bioremediation, carrier selection, and immobilization methods, as well as the pros and cons of immobilization and its prospects.