A fast and efficient method for total DNA extraction from soil filamentous fungi

Wilson Huanca-Mamani; Ricardo Salvatierra Martínez; Germán Sepúlveda-Chavera

doi:10.4067/S0718-34292014000200010

A fast and efficient method for total DNA extraction from soil filamentous fungi

Wilson Huanca-Mamani
, Ricardo Salvatierra Martínez
, Germán Sepúlveda-Chavera

Department of Environmental Resources

Universidad de Tarapacá

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.

Translated title of the contribution	Un método rápido y eficiente para la extracción de ADN total de hongos filamentosos del suelo
Original language	English
Pages (from-to)	75-78
Number of pages	4
Journal	Idesia
Volume	32
Issue number	2
DOIs	https://doi.org/10.4067/S0718-34292014000200010
State	Published - May 2014

Keywords

DNA extraction
Fungi
PCR
Soil

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10.4067/S0718-34292014000200010

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title = "A fast and efficient method for total DNA extraction from soil filamentous fungi",

abstract = "Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.",

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AU - Huanca-Mamani, Wilson

AU - Salvatierra Martínez, Ricardo

AU - Sepúlveda-Chavera, Germán

PY - 2014/5

Y1 - 2014/5

N2 - Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.

AB - Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.

KW - DNA extraction

KW - Fungi

KW - PCR

KW - Soil

UR - https://www.scopus.com/pages/publications/84903436961

U2 - 10.4067/S0718-34292014000200010

DO - 10.4067/S0718-34292014000200010

M3 - Article

AN - SCOPUS:84903436961

SN - 0073-4675

VL - 32

SP - 75

EP - 78

JO - Idesia

JF - Idesia

IS - 2

ER -

A fast and efficient method for total DNA extraction from soil filamentous fungi

Abstract

Keywords

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